dacB [2019-08-07 09:06:58]

sporulation-specific penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase

Locus: BSU_23190

Isoelectric point: 9.73

Molecular weight: 42.92 kDa

Protein length: 382 aa

Gene length: 1149 bp

Function: carboxypeptidase

Product: penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Penicillin-binding proteins
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,423,383 2,424,531

Phenotypes of a mutant

• inactivation of dacB reduces sporulation efficiency to 19.3% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• modifies degree of cross-linking of glycan strands in peptidoglycan

Protein family

• Peptidase S11 family (according to UniProt)

Paralogous protein(s)

• DacF

Structure

• 3MFD (beta-lactamase superfamily domain)

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: dacB-spmA-spmB
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

Biological materials

Mutant

• MGNA-A098 (dacB::erm), available at the NBRP B. subtilis, Japan
• 1A744 ( dacB::cat), PubMed, available at BGSC
• 1A745 ( dacB::cat), PubMed, available at BGSC
• BKE23190 (dacB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTGCTCACGTCCTTG, downstream forward: _UP4_CTGAATGCGGCAGGCGGAGC
• BKK23190 (dacB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTGCTCACGTCCTTG, downstream forward: _UP4_CTGAATGCGGCAGGCGGAGC

References

Reviews

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Original publications

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